Synthesis and Biological Evaluation of Polyethylene Glycol-Hyaluronic Acid-Melphalan as a Potential Therapy for Tumor

Melphalan has been shown to effectively improve cancers by inhibiting deoxyribonucleic acid synthesis. However, it some obvious drawbacks such as short plasma half-life, non-target selectivity and serious adverse reactions. Because of these shortcomings, its clinical application is limited. In the present research work, we have tried obtain polyethylene glycol-hyaluronic modifying hyaluronic with activated glycol; was then connected melphalan prepare acid-melphalan prodrug. Polyethylene obtained glycol The prodrug generates micelles self-assembly in aqueous solution characterized nuclear magnetic resonance infrared spectroscopy. Changes tumor cell morphology cytotoxicity were observed an inverted microscope. Dynamic light scattering transmission electron microscope showed that formed micelle evenly distributed 100-130 nm. Tumor cells treated (10−7 10−6 mol/l respectively) significant changes proliferation compared control group (p<0.05, p<0.01) half-maximal inhibitory concentration values 0.53 μM/48 h. (10−6 mol/l) condensed rounded, aggregated into clumps, number significantly reduced at both 24 h 48 after treatment. synthesized successfully fourier-transform spectroscopy, proton resonance, dynamic It can form average size about demonstrated natural, biocompatible biodegradable particles be used drug delivery system found this compound play potent role on cells, indicated could very useful release targeted drug.